Nucleocapsid amino acids 211 to 254, in particular, tetrad glutamines, are essential for the interaction between the nucleocapsid and membrane proteins of SARS-associated coronavirus.
Identifieur interne : 003E00 ( Main/Exploration ); précédent : 003D99; suivant : 003E01Nucleocapsid amino acids 211 to 254, in particular, tetrad glutamines, are essential for the interaction between the nucleocapsid and membrane proteins of SARS-associated coronavirus.
Auteurs : Xiaonan Fang [République populaire de Chine] ; Lin-Bai Ye ; Yijuan Zhang ; Baozong Li ; Shanshan Li ; Lingbao Kong ; Yuhua Wang ; Hong Zheng ; Wei Wang ; Zhenghui WuSource :
- Journal of microbiology (Seoul, Korea) [ 1225-8873 ] ; 2006.
Descripteurs français
- KwdFr :
- Acide glutamique (génétique), Acide glutamique (métabolisme), Glutamine (génétique), Glutamine (métabolisme), Liaison aux protéines (génétique), Nucléocapside (métabolisme), Peptides (génétique), Peptides (métabolisme), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Sites de fixation (génétique), Substitution d'acide aminé (génétique), Virus du SRAS (génétique), Virus du SRAS (métabolisme), Électrophorèse sur gel de polyacrylamide.
- MESH :
- génétique : Acide glutamique, Glutamine, Liaison aux protéines, Peptides, Protéines nucléocapside, Sites de fixation, Substitution d'acide aminé, Virus du SRAS.
- métabolisme : Acide glutamique, Glutamine, Nucléocapside, Peptides, Protéines nucléocapside, Virus du SRAS.
- Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Amino Acid Substitution (genetics), Binding Sites (genetics), Electrophoresis, Polyacrylamide Gel, Glutamic Acid (genetics), Glutamic Acid (metabolism), Glutamine (genetics), Glutamine (metabolism), Nucleocapsid (metabolism), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Peptides (genetics), Peptides (metabolism), Protein Binding (genetics), SARS Virus (genetics), SARS Virus (metabolism).
- MESH :
- chemical , genetics : Glutamic Acid, Glutamine, Nucleocapsid Proteins, Peptides.
- genetics : Amino Acid Substitution, Binding Sites, Protein Binding, SARS Virus.
- chemical , metabolism : Glutamic Acid, Glutamine, Nucleocapsid, Nucleocapsid Proteins, Peptides, SARS Virus.
- Electrophoresis, Polyacrylamide Gel.
Abstract
GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.
PubMed: 17082754
Affiliations:
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Le document en format XML
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type="province">Hubei</region></placeName></affiliation></author><author><name sortKey="Ye, Lin Bai" sort="Ye, Lin Bai" uniqKey="Ye L" first="Lin-Bai" last="Ye">Lin-Bai Ye</name></author><author><name sortKey="Zhang, Yijuan" sort="Zhang, Yijuan" uniqKey="Zhang Y" first="Yijuan" last="Zhang">Yijuan Zhang</name></author><author><name sortKey="Li, Baozong" sort="Li, Baozong" uniqKey="Li B" first="Baozong" last="Li">Baozong Li</name></author><author><name sortKey="Li, Shanshan" sort="Li, Shanshan" uniqKey="Li S" first="Shanshan" last="Li">Shanshan Li</name></author><author><name sortKey="Kong, Lingbao" sort="Kong, Lingbao" uniqKey="Kong L" first="Lingbao" last="Kong">Lingbao Kong</name></author><author><name sortKey="Wang, Yuhua" sort="Wang, Yuhua" uniqKey="Wang Y" first="Yuhua" last="Wang">Yuhua Wang</name></author><author><name sortKey="Zheng, Hong" sort="Zheng, Hong" uniqKey="Zheng H" first="Hong" last="Zheng">Hong Zheng</name></author><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name></author><author><name sortKey="Wu, Zhenghui" sort="Wu, Zhenghui" uniqKey="Wu Z" first="Zhenghui" last="Wu">Zhenghui Wu</name></author></analytic><series><title level="j">Journal of microbiology (Seoul, Korea)</title><idno type="ISSN">1225-8873</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Substitution (genetics)</term><term>Binding Sites (genetics)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Glutamic Acid (genetics)</term><term>Glutamic Acid (metabolism)</term><term>Glutamine (genetics)</term><term>Glutamine (metabolism)</term><term>Nucleocapsid (metabolism)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (metabolism)</term><term>Peptides (genetics)</term><term>Peptides (metabolism)</term><term>Protein Binding 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Polyacrylamide Gel</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><noCountry><name sortKey="Kong, Lingbao" sort="Kong, Lingbao" uniqKey="Kong L" first="Lingbao" last="Kong">Lingbao Kong</name><name sortKey="Li, Baozong" sort="Li, Baozong" uniqKey="Li B" first="Baozong" last="Li">Baozong Li</name><name sortKey="Li, Shanshan" sort="Li, Shanshan" uniqKey="Li S" first="Shanshan" last="Li">Shanshan Li</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Wang, Yuhua" sort="Wang, Yuhua" uniqKey="Wang Y" first="Yuhua" last="Wang">Yuhua Wang</name><name sortKey="Wu, Zhenghui" sort="Wu, Zhenghui" uniqKey="Wu Z" first="Zhenghui" last="Wu">Zhenghui Wu</name><name sortKey="Ye, Lin Bai" sort="Ye, Lin Bai" uniqKey="Ye L" first="Lin-Bai" last="Ye">Lin-Bai Ye</name><name sortKey="Zhang, Yijuan" sort="Zhang, Yijuan" uniqKey="Zhang Y" first="Yijuan" last="Zhang">Yijuan Zhang</name><name sortKey="Zheng, Hong" sort="Zheng, Hong" uniqKey="Zheng H" first="Hong" last="Zheng">Hong Zheng</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Fang, Xiaonan" sort="Fang, Xiaonan" uniqKey="Fang X" first="Xiaonan" last="Fang">Xiaonan Fang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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